IMMUNIZATION AGAINST BRUCELLA INFECTION II

Immunization against brucella infection. II. Effectiveness of a streptomycin-dependent strain of brucella melitensis.

In previous reports (Elberg and Herzberg, 1953; Herzberg and Elberg, 1953) the isolation and characteristics of a streptomycin-dependent mutant of Brucella melitenis were described.2 The streptomycin-dependent strain retained the biochemical and antigenic characteristics of the virulent parent strain but differed in a requirement for streptomycin for its growth. Growth in vivo was not influence...

Immunisation of Adult Sheep Against Brucella Infection

Immunization of Mice with Recombinant Brucella abortus Organic Hydroperoxide Resistance (Ohr) Protein Protects Against a Virulent Brucella abortus 544 Infection.

In this study, the Brucella abortus ohr gene coding for an organic hydroperoxide resistance protein (Ohr) was cloned into a maltose fusion protein expression system (pMAL), inserted into Escherichia coli, and purified, and its immunogenicity was evaluated by western blot analysis using Brucella-positive mouse sera. The purified recombinant Ohr (rOhr) was treated with adjuvant and injected intra...

Immunization of BALB/c mice with Brucella abortus 2308ΔwbkA confers protection against wild-type infection

Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the Brucella (B.) abortus 2380 wbkA mutant (2308ΔwbkA) and evaluated its virulence. The survival of 2308Δwbk...

Partial protection against Brucella infection in mice by immunization with nonpathogenic alphaproteobacteria.

Previous findings indicate that Brucella antigens and those from nonpathogenic alphaproteobacteria (NPAP) are cross-recognized by the immune system. We hypothesized that immunization with NPAP would protect mice from Brucella infection. Mice were immunized subcutaneously with heat-killed Ochrobactrum anthropi, Sinorhizobium meliloti, Mesorhizobium loti, Agrobacterium tumefaciens, or Brucella me...